20+ How To Calculate Kcat. To determine kcat, one must obviously know the vmax at a particular concentration of enzyme, but the beauty of the term is that it is a measure of velocity independent of enzyme. $$k_ {cat} = frac {v_ {max}} { [e]_t}$$ km is the michaelis constant of.

The equation requires calculating the vmax, which is the maximum rate of conversion. The turnover number (kcat) is a key parameter in enzyme. To calculate the kcat value of an enzyme reaction, you need to divide the maximum velocity (vmax) by the total enzyme concentration ( [e]total).








